Measurement

Part:BBa_K1381009

Designed by: Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg   Group: iGEM14_Uppsala   (2014-10-02)

yenbox_J23113-B0032-GFP

This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K1381004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.


The BioBricks that were used for assembling this construct was BBa_K1381001 (yenbox_J23113) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032). To read more about the Yen system and for the results from the characterisation experiments, see BBa_K1381004 (B0034-YenR) or BBa_K1381001 (yenbox_J23113).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751


[edit]
Categories
Parameters
None